mouse monoclonal antibodies against cd34  (Biocare Medical)

Journal: Frontiers in Neuroanatomy

Article Title: Arterial supply and morphological characteristics of sympathetic neurons in the human superior cervical ganglion

doi: 10.3389/fnana.2024.1372180

Figure Lengend Snippet: Arterial supply and micromorphological characteristics of the superior cervical sympathetic ganglion (SCSG). (A) SCSG (1) with borders indicated by small arrows receives three branches (large arrows) coming from the ascending pharyngeal artery (2), posterior to the internal carotid artery (3), vagus nerve (4) and internal jugular vein (5); posterior pharyngeal wall (6); occipital condyle, transected (7) (view from the back after disarticulation of the head and reflection of the pharyngeal wall anteriorly from the vertebral column). (B) SCSG specimen stained with hematoxilin and eosin (H&E) stain. (C) SCSG specimen stained with the Masson trichrome method. (D) SCSG specimen stained with silver staining method. (E) SCSG specimen and (F) small-magnification view showing vascular supply of the ganglionic tissue (CD34 immunostaining). (G) SCSG specimen and (H) small-magnification view showing mast cells related to the ganglionic tissue (mast cell tryptase immunostaining).

Article Snippet: The slices underwent the immunostaining by the incubation with the following mouse monoclonal primary antibodies; against CD34 (DAKO A/S, Denmark M 7165, 1:25), and anti-mast cell tryptase (DAKO A/S, Denmark M 7052, 1:100).

Techniques: Staining, Silver Staining, Immunostaining

Journal: Frontiers in Neuroanatomy

Article Title: Arterial supply and morphological characteristics of sympathetic neurons in the human superior cervical ganglion

doi: 10.3389/fnana.2024.1372180

Figure Lengend Snippet: (A–C) Immunohistochemical characteristics of the superior cervical sympathetic ganglion (SCSG) blood supply (CD34 immunostaining), and (E–H) mast cells related to the human SCSG neurons (mast cell tryptase immunostaining). (A) Slender network of intraganglionic microvessels surrounding the ganglionic neurons of SCSG and parallel microvessels within the fascicle of axons and fibrous tissue. (B) Delicate plexiform microvessels of the upper part of SCSG fill the interneuronal spaces. (C) Dense network of microvessels of the lower part of SCSG covering the satellite glial cells and the deeper neurons. (D) Detail of higher magnification of the ganglionic capillary plexus injected with India ink and cleared in methyl salicylate. (E) Low-magnification of the upper part of SCSG specimen and (F) high-magnification view showing mast cells (arrows) related to the neurons (mast cell tryptase immunostaining). (G) Low-magnification of the lower part of SCSG specimen and (H) high-magnification view showing mast cells (arrows) related to the ganglionic tissue (mast cell tryptase immunostaining). (I) Tryptase-positive mast cells (yellow arrows) always in close vicinity to blood vessels (red arrows). (J) Numerous mast cells (yellow arrows) found between the axons of the internal carotid nerve at the exit from SCSG.

Article Snippet: The slices underwent the immunostaining by the incubation with the following mouse monoclonal primary antibodies; against CD34 (DAKO A/S, Denmark M 7165, 1:25), and anti-mast cell tryptase (DAKO A/S, Denmark M 7052, 1:100).

Techniques: Immunohistochemical staining, Immunostaining, Injection

Journal: bioRxiv

Article Title: Factor XI localization in human deep venous thrombus and function of activated factor XI on venous thrombus formation and hemostasis in rabbit

doi: 10.1101/2023.09.19.558346

Figure Lengend Snippet: A . Representative immunofluorescent images of fresh component of DVT. Upper row shows CF488-labeled FXI (green), CD568-labeled fibrin (red), and merged images. Middle row shows CF488-labeled FXI, CD568-labeled von Willebrand factor (VWF), and merged images. Lower row shows CF488-labeled FXI, CD568-labeled glycophorin A, and merged images. FXI was closely localized with fibrin, and partly localized with VWF, a marker of platelets. Glycophorin A, a marker of erythrocytes, is predominantly surrounded by FXI. B . Representative immunohistochemical images in fresh and organizing areas of DVT. The organizing areas were confirmed by presence of CD34 immunopositive cells, corresponding to endothelialization/ organization, while the fresh area was confirmed by absence of CD34 immunopositive cells. FXI localized fibrin– and erythrocyte-rich fresh area. In the fresh area, aggregated clusters of platelets and sparsely distributed neutrophils are present. Inset in CD66b represents a high-magnification image of the dashed square. In the organizing area, immunoreaction for FXI, fibrin, glycoprotein IIb/IIIa (GPIIb/IIIa), glycophorin A, or CD66b is modest or focal. C . Immunopositive area for FXI, fibrin, GPIIb/IIIa, and glycophorin A, and CD66b-immunopositive cell density in non-organizing (CD34-immunonegative) and organizing (CD34-immunopositive) areas. (Mann-Whitney U-test)

Article Snippet: Briefly, consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio), fibrin (Millipore), anti-platelet glycoprotein (GP) IIb/IIIa (Sheep monoclonal, Affinity Biologicals Inc., Ancaster, Canada), glycophorin A (Dako/Agilent), and CD66b (mouse monoclonal, clone 6/40c; BioLegend, San Diego, CA, USA).

Techniques: Labeling, Marker, Immunohistochemistry, MANN-WHITNEY

Journal: Vascular medicine (London, England)

Article Title: Incremental Effects of Diabetes Mellitus and Chronic Kidney Disease in Medial Arterial Calcification: Synergistic Pathways for Peripheral Artery Disease Progression

doi: 10.1177/1358863X19842276

Figure Lengend Snippet: Histopathological profiles in the groups

Article Snippet: Formalin fixed paraffin embedded sections were stained with a mouse monoclonal antibody against human CD68/CD3 (Dako Inc, CA) linked to a red chromogen for inflammation, and a mouse monoclonal antibody against human CD34 (Dako Inc, CA) for neovascularization linked to a blue chromogen using double-label immunohistochemistry as previously published 12 .

Techniques: Staining, Expressing

Journal: Proteome Science

Article Title: Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells

doi: 10.1186/1477-5956-9-3

Figure Lengend Snippet: Establishment of primary hair bulge cell culture . (A) Immunohistological staining revealed a large number of CD34 + cells (arrows) present in the vicinity of the hair bulge (HB) inferior to sebaceous glands (SG). (B) Showing an isolated hair follicle from mouse vibrissal hair. The hair bulge was dissected (extent indicated by the dotted lines) from the follicle and used for explant culture. (C) Showing cells that have migrated out from the bulge explant to form colonies of cells all around the explant. (D) Showing the appearance of HBPCs following purification with anti-CD34 conjugated magnetic beads.

Article Snippet: Primary antibodies used were mouse monoclonal antibodies against CD34 (Lab Vision, CA, USA), K14 (Calbiochem, CA, USA), active β-catenin (Millipore, CA, USA), GATA4 (Santa Cruz Biotechnology, CA, USA), sarcomeric myosin heavy chain (Developmental Studies Hybridoma Bank, Iowa, IA, USA), Cardiac-specific troponin I (Lab Vision) and Islet1 (R & D Systems).

Techniques: Cell Culture, Staining, Isolation, Purification, Magnetic Beads

Journal: Proteome Science

Article Title: Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells

doi: 10.1186/1477-5956-9-3

Figure Lengend Snippet: Primers for semi-quantitative RT-PCR analysis

Article Snippet: Primary antibodies used were mouse monoclonal antibodies against CD34 (Lab Vision, CA, USA), K14 (Calbiochem, CA, USA), active β-catenin (Millipore, CA, USA), GATA4 (Santa Cruz Biotechnology, CA, USA), sarcomeric myosin heavy chain (Developmental Studies Hybridoma Bank, Iowa, IA, USA), Cardiac-specific troponin I (Lab Vision) and Islet1 (R & D Systems).

Techniques:

Journal: Proteome Science

Article Title: Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells

doi: 10.1186/1477-5956-9-3

Figure Lengend Snippet: Characterization of CD34 + HBPCs . (A-B) Immunofluorescent staining showed HBPCs specifically expressed established HBPCs surface markers Keratin 14 (K14) and 15 (K15). (C) Semi-quantitative RT-PCR analysis confirmed that the HBPCs expressed K14. In addition, HBPCs also expressed K5 and Snail and Sox2. (D) In contrast, dermal fibroblasts (DFs) isolated from adjacent to the hair bulge did not express K14 and K5. β-actin served as an internal control.

Article Snippet: Primary antibodies used were mouse monoclonal antibodies against CD34 (Lab Vision, CA, USA), K14 (Calbiochem, CA, USA), active β-catenin (Millipore, CA, USA), GATA4 (Santa Cruz Biotechnology, CA, USA), sarcomeric myosin heavy chain (Developmental Studies Hybridoma Bank, Iowa, IA, USA), Cardiac-specific troponin I (Lab Vision) and Islet1 (R & D Systems).

Techniques: Staining, Quantitative RT-PCR, Isolation

Journal: Proteome Science

Article Title: Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells

doi: 10.1186/1477-5956-9-3

Figure Lengend Snippet: Developmental potential of HBPCs . (A) Adipogenic potential of HBPCs was examined. Oil Red O dye staining revealed the presence of adipocytes (arrows) in the HBPC cultures, 3 weeks after adipogenic-inducing medium treatment. Approximately 27 ± 3% of the induced cells were adipocytes. (B) Scanning electron micrograph showing a representative adipocyte that have formed in the cultures. The cytoplasm of these cells was filled with numerous small lipid vesicles (arrows). (C) Semi-quantitative RT-PCR analysis revealed that after the HBPCs were incubated with adipogenic-inducing medium for 3 weeks, CD34 and Nestin (HBPC marker) expression were inhibited while PPAR-γ (adipocyte marker) expression was induced. β-actin served as an internal control. (D) Osteogenic potential of HBPCs was also examined. Alizarin Red S staining revealed the presence of osteocytes (arrows) in the cultures, after 3 weeks osteogenic-inducing medium induction. Approximately 31 ± 3% of the treated cells have transdifferentiated into osteocytes (arrows). (E) Transmission electron micrograph showing a representative osteocyte that has transdifferentiated from the HBPCs. These cells were capable of secreting bone matrix (arrows). (F) Semi-quantitative RT-PCR analysis revealed that after HBPCs were incubated in osteogenic-inducing medium for 3 weeks, CD34 and Nestin expressions were inhibited while Osteocalcin (bone marker) was activated. β-actin served as an internal control.

Article Snippet: Primary antibodies used were mouse monoclonal antibodies against CD34 (Lab Vision, CA, USA), K14 (Calbiochem, CA, USA), active β-catenin (Millipore, CA, USA), GATA4 (Santa Cruz Biotechnology, CA, USA), sarcomeric myosin heavy chain (Developmental Studies Hybridoma Bank, Iowa, IA, USA), Cardiac-specific troponin I (Lab Vision) and Islet1 (R & D Systems).

Techniques: Staining, Quantitative RT-PCR, Incubation, Marker, Expressing, Transmission Assay

Journal: BMC Cancer

Article Title: Angiogenesis in cancer of unknown primary: clinicopathological study of CD34, VEGF and TSP-1

doi: 10.1186/1471-2407-5-25

Figure Lengend Snippet: High-density of neoplastic vessels highlighted by stained anti-CD34 antibody in a case of a poorly differentiated adenocarcinoma of unknown primary (Original magnification 200, counterstained with hematoxylin).

Article Snippet: Mouse monoclonal antibodies directed against human CD34 antigen (M 7165, Dako) in dilution 1/50 β) VEGF Ab-3 (isoform 121, clone Jh121, Neomarkers) in dilution 1/50 and c) thrombospondin (Mob 315, DBS) in dilution1/50 were used.

Techniques: Staining

Journal: BMC Cancer

Article Title: Angiogenesis in cancer of unknown primary: clinicopathological study of CD34, VEGF and TSP-1

doi: 10.1186/1471-2407-5-25

Figure Lengend Snippet: Assessment of tissue expression of VEGF, TSP-1 and CD34.

Article Snippet: Mouse monoclonal antibodies directed against human CD34 antigen (M 7165, Dako) in dilution 1/50 β) VEGF Ab-3 (isoform 121, clone Jh121, Neomarkers) in dilution 1/50 and c) thrombospondin (Mob 315, DBS) in dilution1/50 were used.

Techniques: Expressing

Journal: BMC Cancer

Article Title: Angiogenesis in cancer of unknown primary: clinicopathological study of CD34, VEGF and TSP-1

doi: 10.1186/1471-2407-5-25

Figure Lengend Snippet: Immunohistochemical expression of CD34 (MVD). VEGF, and TSP-1 and correlations.

Article Snippet: Mouse monoclonal antibodies directed against human CD34 antigen (M 7165, Dako) in dilution 1/50 β) VEGF Ab-3 (isoform 121, clone Jh121, Neomarkers) in dilution 1/50 and c) thrombospondin (Mob 315, DBS) in dilution1/50 were used.

Techniques: Immunohistochemical staining, Expressing

Journal: Breast Cancer Research : BCR

Article Title: Loss of pigment epithelium-derived factor: a novel mechanism for the development of endocrine resistance in breast cancer

doi: 10.1186/bcr3356

Figure Lengend Snippet: Effect of recombinant pigment epithelium-derived factor (rPEDF) on the growth of endocrine-sensitive MCF-7 and endocrine-resistant MCF-7:5C breast cancer cells in vitro and in vivo . (a) MCF-7 and MCF-7:5C cells were treated with increasing concentrations of rPEDF, rPEDF + anti-PEDF antibody (anti-PEDF), or anti-PEDF antibody for 7 days, and cell proliferation was determined using a DNA quantitation kit as described in Materials and methods. Experiments were repeated three times, and data are shown as mean ± standard deviation (SD). * P < 0.001 compared with untreated controls. (b) MCF-7:5C cells were treated with rPEDF, anti-rPEDF, or rPEDF + anti-rPEDF for 72 hours and apoptosis was determined by TUNEL staining. Bar graph: summary of percentage of apoptotic cells counted in five fields from three experiments. Data presented as mean ± SD. (c) Effect of rPEDF on the growth of MCF-7:5C cells in vivo . MCF-7:5C cells were bilaterally injected into the mammary fat pad of ovariectomized nude mice, and when tumors reached an area of 0.1 cm 2 the mice ( n = 15/group) were randomized into two treatment groups: PBS or rPEDF. The mean cross-sectional tumor area was measured up to 30 days. Bar graph: mean cross-sectional tumor area in the control group and the PEDF-treated group. (d) Intratumoral microvessel density (MVD) in tumor tissues was determined by immunohistochemical staining by an endothelial-specific antibody CD34; PBS group (×200) and PEDF group (×200). Quantitative analysis of microvessel density is also shown. Data presented as mean ± SD.

Article Snippet: Tumor sections were incubated with the rat monoclonal antibody against mouse CD34 (BD Pharmingen, San Diego, CA, USA) at 1:100 dilutions at 4°C.

Techniques: Recombinant, Derivative Assay, In Vitro, In Vivo, Quantitation Assay, Standard Deviation, TUNEL Assay, Staining, Injection, Immunohistochemical staining

Journal: ISRN Obesity

Article Title: Downregulation of RelA (p65) by Rapamycin Inhibits Murine Adipocyte Differentiation and Reduces Fat Mass of C57BL/6J Mice despite High Fat Diet

doi: 10.1155/2014/540582

Figure Lengend Snippet: Phenotypic characterization of muADSC cells. FACS analysis of muADSC demonstrating expression of typical surface proteins. Cultured cells used in the experiments were uniformly positive for CD29, CD44, CD73, and Scal-1 and negative for CD14, CD31, CD34, and CD45.

Article Snippet: The primary antibodies (applied in optimal amounts) included a biotin-conjugated rat anti-mouse monoclonal antibody against CD34 and CD29 followed by streptavidin-APC, PE-conjugated mouse anti-Sca-1, and FITC-conjugated mouse anti-CD45 and anti-CD44 (all from BD PharMingen, San Diego, CA).

Techniques: Expressing, Cell Culture